技术参数：

•测量参数包括F0、Ft、Fm、Fm’、QY、QY_Ln、QY_Dn、NPQ、Qp、Rfd、PAR（限PAR型号）、Area、Mo、Sm、PI、ABS/RC等50多个叶绿素荧光参数，及3种给光程序的光响应曲线、3种荧光淬灭曲线、OJIP曲线等

•OJIP–test时间分辨率为10µs（每秒10万次），给出OJIP曲线和26个参数，包括F0、Fj、Fi、Fm、Fv、Vj、Vi、Fm/F0、Fv/F0、Fv/Fm、Mo、Area、Fix Area、Sm、Ss、N、Phi_Po、Psi_o、Phi_Eo、Phi–Do、Phi_Pav、PI_Abs、ABS/RC、TRo/RC、ETo/RC、DIo/RC等

•测量程序：Ft、QY、OJIP、NPQ1、NPQ2、NPQ3、LC1、LC2、LC3、PAR（限PAR型号）、Multi无人值守自动监测

•叶夹类型：FP110/S固定叶夹式、FP110/D分离叶夹式、FP110/P探头式、FP110/X用户定制式

•PAR传感器（限PAR型号）：80º入射角余弦校正，读数单位µmol(photons)/m².s，可显示读数，检测范围400-700 nm

•测量光：每测量脉冲最高0.09µmol(photons)/m².s，10-100%可调

•光化学光：10-1000µmol(photons)/m².s可调

•饱和光：最高3000µmol(photons)/m².s，11-100%可调

•光源：标准配置蓝光455nm，可根据需求配备不同波长的LED光源

•检测器：PIN光电二极管，667–750nm滤波器

•尺寸大小：超便携，手机大小，134×65×33mm（不包括探头），重量仅188g

•数据存储：容量16Mb，可存储149000数据点

•显示与操作：图形化显示，双键操作，待机5分钟自动关闭

•供电：2000mA可充电锂电池，USB充电，可连续工作48小时，低电报警

•工作条件：0–55℃，0–95%相对湿度（无凝结水）

•存贮条件：-10–60℃，0–95％相对湿度（无凝结水）

•通讯方式：蓝牙+USB双通讯模式，蓝牙在20m距离最大传输速度3Mbps

•GPS模块：内置，最高精度1.5m

•软件：FluorPen1.1专用软件，用于数据下载、分析和图表显示，输出Excel数据文件及荧光动力学曲线图，适用于Windows 7及更高操作系统

操作软件与实验结果

应用案例：

2017年4月，美国国家航空航天局（NASA）新一代先进植物培养器（Advanced Plant Habitat，APH）搭载联盟号MS-04货运飞船抵达国际空间站。宇航员使用FluorPen手持仪叶绿素荧光仪在其中开展植物生理学及太空食物种植（growth of fresh food in space）的研究。

参考文献

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2.Ariyarathna, R. a. I. S., Weerasena, S. L. & Beneragama, C. K. Application of Polyphasic OJIP Chlorophyll Fluorescent Transient Analysis as an Indicator for Testing of Seedling Vigour of Common Bean (Phaseolus vulgaris L.). Tropical Agricultural Research 31, 106–115 (2020).

3.Prity, S. A. et al. Arbuscular mycorrhizal fungi mitigate Fe deficiency symptoms in sorghum through phytosiderophore-mediated Fe mobilization and restoration of redox status. Protoplasma (2020) doi:10.1007/s00709-020-01517-w.

4.Rahman, M. A. et al. Arbuscular Mycorrhizal Symbiosis Mitigates Iron (Fe)-Deficiency Retardation in Alfalfa (Medicago sativa L.) Through the Enhancement of Fe Accumulation and Sulfur-Assisted Antioxidant Defense. International Journal of Molecular Sciences 21, 2219 (2020).

5.Vitorino, L. C. et al. Biocontrol Potential of Sclerotinia sclerotiorum and Physiological Changes in Soybean in Response to Butia archeri Palm Rhizobacteria. Plants 9, 64 (2020).

6.Aalifar, M. et al. Blue Light Improves Vase Life of Carnation Cut Flowers Through Its Effect on the Antioxidant Defense System. Front. Plant Sci. 11, 511 (2020).

7.Muthusamy, M., Yoon, E. K., Kim, J. A., Jeong, M.-J. & Lee, S. I. Brassica Rapa SR45a Regulates Drought Tolerance via the Alternative Splicing of Target Genes. Genes 11, 182 (2020).

8.Muthusamy, M., Kim, J. Y., Yoon, E. K., Kim, J. A. & Lee, S. I. BrEXLB1, a Brassica rapa Expansin-Like B1 Gene Is Associated with Root Development, Drought Stress Response, and Seed Germination. Genes 11, 404 (2020).

9.Herritt, M. T. & Fritschi, F. B. Characterization of Photosynthetic Phenotypes and Chloroplast Ultrastructural Changes of Soybean (Glycine max) in Response to Elevated Air Temperatures. Front. Plant Sci. 11, (2020).

10.Kasampalis, D. S., Tsouvaltzis, P. & Siomos, A. S. Chlorophyll fluorescence, non-photochemical quenching and light harvesting complex as alternatives to color measurement, in classifying tomato fruit according to their maturity stage at harvest and in monitoring postharvest ripening during storage. Postharvest Biology and Technology 161, 111036 (2020).

11.Soares, J. S., Santiago, E. F. & Sorgato, J. C. Conservation of Schomburgkia crispa Lindl. (Orchidaceae) by reintroduction into a fragment of the Brazilian Cerrado. Journal for Nature Conservation 53, 125754 (2020).

12.Poblete, T. et al. Detection of Xylella fastidiosa infection symptoms with airborne multispectral and thermal imagery: Assessing bandset reduction performance from hyperspectral analysis. ISPRS Journal of Photogrammetry and Remote Sensing 162, 27–40 (2020).

13.Chiluwal, A. et al. Deterioration of ovary plays a key role in heat stress-induced spikelet sterility in sorghum. Plant, Cell & Environment 43, 448–462 (2020).

14.Maai, E., Nishimura, K., Takisawa, R. & Nakazaki, T. Diurnal changes in chloroplast positioning and photosynthetic traits of C4 grass finger millet. Plant Production Science 0, 1–13 (2020).

15.De Micco, V. et al. Dust accumulation due to anthropogenic impact induces anatomical and photochemical changes in leaves of Centranthus ruber growing on the slope of the Vesuvius volcano. Plant Biol J 22, 93–102 (2020).

附：OJIP参数及计算公式

Bckg = background 

Fo: = F50µs; fluorescence intensity at 50 µs 

Fj: = fluorescence intensity at j-step (at 2 ms) 

Fi: = fluorescence intensity at i-step (at 60 ms) 

Fm: = maximal fluorescence intensity 

Fv: = Fm - Fo (maximal variable fluorescence) 

Vj = (Fj - Fo) / (Fm - Fo) 

Fm / Fo = Fm / Fo 

Fv / Fo = Fv / Fo 

Fv / Fm = Fv / Fm 

Mo = TRo / RC - ETo / RC 

Area = area between fluorescence curve and Fm 

Sm = area / Fm - Fo (multiple turn-over) 

Ss = the smallest Sm turn-over (single turn-over) 

N = Sm . Mo . (I / Vj) turn-over number QA 

Phi_Po = (I - Fo) / Fm (or Fv / Fm) 

Phi_o = I - Vj 

Phi_Eo = (I - Fo / Fm) . Phi_o 

Phi_Do = 1 - Phi_Po - (Fo / Fm) 

Phi_Pav = Phi_Po - (Sm / tFM); tFM = time to reach Fm (in ms) 

ABS / RC = Mo . (I / Vj) . (I / Phi_Po) 

TRo / RC = Mo . (I / Vj) 

ETo / RC = Mo . (I / Vj) . Phi_o) 

DIo / RC = (ABS / RC) - (TRo / RC)